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STATA Corporation
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Becton Dickinson
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Cardiff Software
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Dynamic Microsystems Inc
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Image Search Results
Journal: The Journal of Neuroscience
Article Title: Tumor Necrosis Factor α But Not Interleukin 1β Mediates Neuroprotection in Response to Acute Nitric Oxide Excitotoxicity
doi: 10.1523/JNEUROSCI.4032-05.2006
Figure Lengend Snippet: Three-dimensional representation of the volume of neuronal cell death, as measured by FJB-positive staining in wild-type (WT), IL-1β, TNF-α, and IL-1β/TNF-α double (Dbl) KO mice killed 4 d after an intraparenchymal injection of the excitotoxin SNP. A, These 3D reconstructions of FJB-positive regions were performed using a C-80 Nikon microscope and super-high-pressure mercury lamp (Nikon) fitted with a Retiga EXi Fast digital camera (QImaging) feeding to a Precision 660 workstation (Dell Computer). The FJB-positive regions were traced on a Wacom pen tablet using the Neurolucida stereological software package (version 6.02.1; MicroBrightField), and 3D reconstruction and volume determination were compiled using Neuroexplorer software (version 4.01.1; MicroBrightField). Please note the larger volumes of FJB in the TNF and Dbl KO animals compared with that of wild-type and IL-1 KO mice. B, Quantitative analysis of the FJB volume in the brains of wild-type, IL-1, TNF, and Dbl KO mice over time. Please note the rapid appearance of neuronal cell death 6 h after SNP injection and the marked spread of FJB staining (**p < 0.01 compared with wild type and IL-1 KO; data presented as mean ± SEM) in the CNS of TNF and Dbl KO 4 d after the single SNP infusion. For photomicrographs of the FJB staining, refer to Figures Figures11 and and88.
Article Snippet: The FJB-positive regions were traced on a Wacom (Vancouver, WA) pen tablet using the
Techniques: Staining, Injection, Microscopy, Software